Background signals in stimulated Raman scattering microscopy and current solutions to avoid them

Stimulated Raman scattering (SRS) microscopy has gained popularity in recent years due to its linearity molecule concentration and laser intensity, the lack of nonresonant background that affects analogous technique, coherent anti-Stokes scattering. However, SRS is not a background-free technique. In fact, there are other optical processes – nonlinear transient absorption can be detrimental contrast sensitivity microscopy. this review, we provide description these competing present an up-to-date current solutions minimize their effect on measurements.